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Quantification of Thrips Damage Using Ilastik and ImageJ Fiji
Isabella G S Visschers1, Nicole M Van Dam1,2,3, Janny L Peters4
1Department of Molecular Interaction Ecology, Institute for Water and Wetland Research, Radboud University, 6525 AJ Nijmegen, The Netherlands.
Abstract:
Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available software programs Ilastik and ImageJ. The protocol was developed in order to screen over 40 Capsicum lines for resistance against Frankliniella occidentalis (Western Flower Thrips) and Thrips tabaci (Onion thrips).
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