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Updated: Oct 27, 2025
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
Published on: November 14, 2018
Method for CRISPR/Cas9 Mutagenesis in Candida albicans
1Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA.
This study presents a rapid CRISPR-based method for creating and analyzing gene deletion mutants in Candida albicans, a common fungal pathogen. The protocol enables efficient isolation and characterization of mutants within nine days.
Area of Science:
- Medical Mycology
- Molecular Biology
- Gene Editing Technologies
Background:
- Candida albicans is a significant human fungal pathogen.
- CRISPR gene editing has advanced genetic studies in C. albicans.
Purpose of the Study:
- To provide a detailed protocol for constructing and analyzing C. albicans deletion mutants.
- To leverage CRISPR technology for efficient genetic manipulation in C. albicans.
Main Methods:
- Utilizing plasmids for high copy guide RNA expression.
- Employing synthetic duplex 23mer guide oligodeoxynucleotides.
- Using codon-optimized Cas9 in C. albicans strains.
Main Results:
- Successful construction and analysis of C. albicans deletion mutants.
- Demonstration of a streamlined protocol for genetic analysis.
- Isolation and characterization of deletion strains achieved within nine days.
Conclusions:
- The described CRISPR-based protocol offers a rapid and effective method for generating C. albicans deletion mutants.
- This advancement facilitates further genetic research into C. albicans pathogenesis and antifungal development.
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