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Related Concept Videos

Chromatin Packaging02:21

Chromatin Packaging

17.9K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
17.9K
Chromatin Packaging01:32

Chromatin Packaging

18.0K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
18.0K
Nucleosome Remodeling02:54

Nucleosome Remodeling

10.0K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
10.0K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.6K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.6K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

6.4K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
6.4K

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Related Experiment Video

Updated: Oct 27, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
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The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

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A Method for Extracting the Nuclear Scaffold from the Chromatin Network.

Junjie Chen1, Boon Heng Dennis Teo1, Jinhua Lu1

  • 1Department of Microbiology, Yong Loo Lin School of Medicine, Immunology Program, National University of Singapore, Singapore, Singapore.

Bio-Protocol
|July 21, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a new protocol to extract the nuclear scaffold, revealing insights into chromatin organization. This method aids in understanding DNA packaging within the cell nucleus.

Keywords:
ChromatinExtractNucleiNucleophosmin-1Scaffold

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A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
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Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol
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Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cells contain large DNA polymers organized into chromatin within the nucleus.
  • The precise mechanism of chromatin compaction and the role of the nuclear scaffold are not fully understood.
  • Previous studies lacked methods for comprehensive nuclear scaffold extraction and analysis.

Purpose of the Study:

  • To develop a simple and reproducible protocol for extracting the nuclear scaffold.
  • To investigate the composition and function of the nuclear scaffold in chromatin organization.
  • To provide insights into the higher-order structure of DNA within the nucleus.

Main Methods:

  • Generation of pure nuclei.
  • Treatment with Triton X-100 to create envelope-depleted nuclei (TxN).
  • Extraction of scaffold components using a 500 mM NaCl solution in a sucrose buffer, yielding TxNE.

Main Results:

  • The developed protocol successfully released scaffold components from chromatin.
  • The extraction process resulted in the liberation of nuclear chromatins into extended, parallel fibers, indicating successful scaffold removal.
  • The extract (TxNE) contains crucial components of the nuclear scaffold.

Conclusions:

  • The new protocol provides an inclusive and reproducible method for nuclear scaffold extraction.
  • This method facilitates biochemical and proteomic studies of the nuclear scaffold.
  • Understanding the nuclear scaffold is essential for elucidating chromatin organization and DNA packaging within the nucleus.