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Isolation and Maintenance of Murine Embryonic Striatal Neurons
Luana Naia1, A Cristina Rego1,2
1CNC-Center for Neuroscience and Cell Biology, University of Coimbra (UC), Coimbra, Portugal.
Bio-Protocol
|July 21, 2021
Abstract:
Primary cultures of murine striatal neurons are widely used to explore cellular mechanisms in neurobiology, including brain diseases. Here we describe a detailed and standardized protocol to dissect and culture embryonic murine striatal neurons GABA-positive/DARPP-32-positive for 12 days in vitro, when they show good neuronal cell connectivity and the presence of dendritic spines, which reflects the maturation of the network.
