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Related Experiment Video

Updated: Oct 27, 2025

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

Published on: March 3, 2016

A simple, efficient, and reliable endoderm differentiation protocol for human embryonic stem cells using crotonate.

Yi Fang¹, Xiaoling Li¹

¹Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.

|July 21, 2021

Summary

This study introduces a simple protocol using crotonate to efficiently differentiate human embryonic stem cells (hESCs) into endoderm. This method enhances differentiation and reduces reagent needs.

Keywords:

Cell Biology Cell Differentiation Cell culture Stem Cells Tissue Engineering

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Last Updated: Oct 27, 2025

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

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Published on: March 3, 2016

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Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

Published on: March 27, 2012

Area of Science:

Stem cell biology
Epigenetics
Developmental biology

Background:

Human embryonic stem cell (hESC) differentiation typically requires extensive training and experience.
Inducing specific cell lineages like endoderm is crucial for regenerative medicine and disease modeling.

Purpose of the Study:

To develop a simplified and highly efficient protocol for endoderm differentiation of hESCs.
To investigate the role of crotonate in enhancing hESC differentiation.

Main Methods:

Utilized crotonate, a precursor for histone crotonylation, in endoderm differentiation media for hESCs.
Optimized the addition of crotonate to standard endoderm differentiation media.

Main Results:

The addition of crotonate significantly increased the efficiency of endoderm differentiation.
The protocol substantially reduced the amount of reagents required for differentiation.

Conclusions:

Crotonate is an effective and efficient inducer of endoderm differentiation in hESCs.
This protocol offers a simplified and cost-effective approach for hESC endoderm differentiation.