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miR-7 regulates the apoptosis of chicken primary myoblasts through the KLF4 gene

X Zhang1, F Chen1, M He1

  • 1College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

British Poultry Science
|July 21, 2021
PubMed

Insights

MicroRNA-7 (miR-7) promotes apoptosis in chicken primary myoblasts by regulating KLF4 expression. This study clarifies miR-7

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Animal Science

Background:

  • MicroRNAs (miRNAs) are crucial regulators of myoblast proliferation, differentiation, and apoptosis.
  • The specific role and mechanism of miR-7 in chicken primary myoblast (CPMs) apoptosis remain largely unknown.

Purpose of the Study:

  • To investigate the effect of miR-7 on apoptosis in CPMs.
  • To elucidate the underlying molecular mechanism involving KLF4.

Main Methods:

  • Transfection of miR-7 mimic and inhibitor in CPMs.
  • Apoptosis marker gene expression analysis (RAF1, Caspase3, Caspase9, Cytc, Fas).
  • Flow cytometry for apoptosis rate assessment.
  • Dual-luciferase reporter assay to identify miR-7 targets.
  • Rescue experiments and gene overexpression/inhibition studies for KLF4.

Main Results:

  • miR-7 mimic significantly increased apoptosis marker gene expression and late apoptosis rate in CPMs, while miR-7 inhibitor showed opposite effects.
  • KLF4 was identified as a direct target gene of miR-7.
  • KLF4 overexpression attenuated miR-7-induced apoptosis, whereas KLF4 inhibition promoted apoptosis in CPMs.
  • Overexpression of KLF4 inhibited early apoptosis, and KLF4 interference promoted it.

Conclusions:

  • miR-7 acts as a pro-apoptotic factor in chicken primary myoblasts.
  • This pro-apoptotic effect is mediated through the regulation of KLF4 expression.
  • The findings provide novel insights into the role of miR-7/KLF4 axis in myoblast apoptosis.
Keywords:
Jinghai yellow chickenKLF4apoptosismiR-7myoblasts

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