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Temperature sensitive R plasmids isolated from Proteus strains.

Y Odakura, H Hashimoto, S Mitsuhashi

Microbiology and Immunology

|November 1, 1977

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Seventeen of 32 R plasmids showed temperature-sensitive inheritance in E. coli. V group R plasmids, like YOR-10, exhibit thermosensitive instability in E. coli at high temperatures.

Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

R plasmids confer antibiotic resistance and can be transferred between bacteria.
Temperature sensitivity in plasmid inheritance can impact bacterial genetics and evolution.
Proteus and E. coli are common bacterial species studied for plasmid dynamics.

Purpose of the Study:

To investigate the temperature sensitivity of R plasmids isolated from Proteus strains in E. coli.
To classify temperature-sensitive R plasmids into incompatibility groups.
To elucidate the mechanisms underlying R plasmid instability at elevated temperatures.

Main Methods:

Isolation and characterization of R plasmids from Proteus strains.
Testing plasmid inheritance and stability in E. coli at different temperatures (25°C and 42°C).

Related Experiment Videos

Classification of plasmids into incompatibility groups (T and V).

Assessment of plasmid-mediated growth and conjugal transfer at high temperatures.

Main Results:

17 out of 32 R plasmids exhibited temperature-sensitive inheritance in E. coli.
Plasmids were classified into incompatibility groups T or V.
T group plasmid Rms273 showed temperature sensitivity in both E. coli and Proteus for growth and transfer.
V group plasmid YOR-10 was stable in Proteus at 42°C but showed 100% loss in E. coli within 4 hours at 42°C.
Conjugal transfer of YOR-10 in E. coli was inhibited at 42°C.

Conclusions:

V group R plasmids demonstrate thermosensitive inheritance instability specifically in E. coli at high temperatures.
The instability is linked to impaired inheritance in progeny cells at elevated temperatures.
Understanding R plasmid thermosensitivity is crucial for managing bacterial genetics and antibiotic resistance spread.