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Identification of aneuploidy in dogs screened by a SNP microarray
Lisa G Shaffer1,2, Bradley Hopp3, Marek Switonski4
1Paw Print Genetics, Genetic Veterinary Sciences, Inc, 220 E Rowan, Suite 220, Spokane, WA, 99207, USA. Lshaffer@pawprintgenetics.com.
Human Genetics
|July 21, 2021
Summary
This study introduces SNP arrays for canine genetic testing, identifying three cases of aneuploidy including monosomy X, trisomy X, and mosaic trisomy 38 in 2053 dogs. This marks the first use of microarrays for canine cytogenetic anomaly detection.
Area of Science:
- Genetics
- Genomics
- Cytogenetics
Background:
- Microarray analysis, particularly SNP arrays, is a robust method for detecting human cytogenetic imbalances like aneuploidy and aneusomy.
- While established in human diagnostics, microarray technology for copy-number evaluation is underutilized in clinical canine genetic testing.
Observation:
- A proprietary SNP array with ~650,000 loci was validated for the domestic dog genome.
- During validation, 2053 dogs were screened, revealing cytogenetic anomalies.
Findings:
- Three cases of canine aneuploidy were identified: monosomy X, trisomy X, and a mosaic trisomy of canine chromosome 38 (CFA38).
- This research is the first to apply microarray analysis for copy-number assessment to detect cytogenetic anomalies in canines.
Implications:
- The implementation of SNP arrays offers a powerful tool for identifying canine chromosomal abnormalities.
- Increased use of microarray analysis in canine genetic testing is expected to uncover further cases of chromosome aneuploidy.
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