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Related Experiment Video

Updated: Oct 27, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

4.4K

Soybean Hairy Root Transformation: A Rapid and Highly Efficient Method.

Jaehyo Song1, Katalin Tóth1,2, Bruna Montes-Luz1

  • 1Division of Plant Sciences, University of Missouri, Columbia, Missouri.

Current Protocols
|July 21, 2021
PubMed
Summary
This summary is machine-generated.

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A new protocol rapidly generates transgenic hairy soybean roots in 18 days, enabling efficient study of root biology and legume-microbe interactions for improved crop traits.

Area of Science:

  • Plant Molecular Biology
  • Genetics
  • Agronomy

Background:

  • Agrobacterium-mediated transformation is crucial for plant genetic studies, particularly in soybean.
  • Stable transgenic soybean generation is time-consuming.
  • Agrobacterium rhizogenes-mediated hairy root transformation offers a faster alternative for root-specific research.

Purpose of the Study:

  • To provide an updated, efficient protocol for generating soybean hairy roots.
  • To demonstrate the utility of composite plants for studying root biology.
  • To facilitate research on soybean agronomic traits and interactions.

Main Methods:

  • Updated protocol for Agrobacterium rhizogenes-mediated hairy root transformation in soybean.
  • Generation of composite plants with transgenic roots and wild-type shoots.
Keywords:
agrobacteriumgene expressionnodulationsoybeantransformationtransgenic roots

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Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation
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Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae
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Last Updated: Oct 27, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
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Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation
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  • Soybean nodulation assays and gene expression analysis (GUS staining).

    • Main Results:

    • Successful generation of soybean hairy roots in as little as 18 days.
    • Cost- and space-effective method for plant research.
    • Demonstrated applicability in nodulation assays and promoter analysis.

    Conclusions:

    • The protocol offers a rapid and efficient method for soybean root transformation.
    • Composite plants are valuable tools for studying root-specific genetic mechanisms.
    • This technique can accelerate research into soybean's molecular and genetic functions.