Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Oct 27, 2025

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.0K

Matrigel® enhances 3T3-L1 cell differentiation.

Chitmandeep Josan1, Sachin Kakar2, Sandeep Raha1,2

  • 1Department of Pediatrics and the Graduate Program in Medical Sciences, McMaster University, Hamilton, ON, Canada.

Adipocyte
|July 21, 2021
PubMed
Summary

Culturing 3T3-L1 cells on Matrigel® enhances adipocyte differentiation and lipid accumulation compared to polystyrene. This bio-gel matrix promotes in vivo-like cellular interactions, improving understanding of adipogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Tubular 3D In Vitro Model of Placental Trophoblasts for Transport Studies.

ACS biomaterials science & engineering·2026
Same author

Prenatal cannabis smoke exposure alters placental development in a murine model of pregnancy.

PloS one·2026
Same author

Development of a tissue-specific bioscaffold for intestinal stem cell culture.

PloS one·2025
Same author

Modeling the Progression of Placental Transport from Early- to Late-Stage Pregnancy by Tuning Trophoblast Differentiation and Vascularization.

Advanced healthcare materials·2023
Same author

Cannabis use during lactation may alter the composition of human breast milk.

Pediatric research·2022
Same author

Prenatal cannabis use associated with adverse birth outcomes.

The Journal of pediatrics·2022

Area of Science:

  • Biomaterials science
  • Cell biology
  • Biotechnology

Background:

  • Bio-gels offer a more in vivo-like extracellular matrix for cell culture.
  • 3T3-L1 cells are a standard model for adipogenesis research.

Purpose of the Study:

  • To investigate the effect of Matrigel® on 3T3-L1 cell proliferation and differentiation.
  • To compare adipogenesis on Matrigel® versus traditional polystyrene surfaces.

Main Methods:

  • 3T3-L1 cells were cultured on Matrigel® and polystyrene surfaces.
  • Gene expression analysis of adipogenic and extracellular matrix markers was performed.
  • Lipid accumulation was quantified.

Main Results:

  • Matrigel® promoted 3T3-L1 cell aggregation and increased mRNA levels of adipogenic factors (PPARγ, C/EBPα, SREBP1) and lipogenic markers (FAS, FABP4, LPL, PLIN1).
Keywords:
3d cell culture3t3-l1 cellsadipocyteadipocyte differentiationadipogenesisadipose tissueextracellular matrixmatrigelrosiglitazone

More Related Videos

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
05:42

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Published on: April 5, 2024

1.2K
IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
10:48

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Published on: November 13, 2023

1.4K

Related Experiment Videos

Last Updated: Oct 27, 2025

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.0K
Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
05:42

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Published on: April 5, 2024

1.2K
IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
10:48

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Published on: November 13, 2023

1.4K
  • Gene expression of extracellular matrix proteins (FN1, COL1A1, COL4A1, COL6, LAM) was reduced on Matrigel®.
  • Matrigel® enhanced lipid accumulation in 3T3-L1 cells, with or without rosiglitazone.

    • Conclusions:

    • Culturing 3T3-L1 cells on Matrigel® significantly enhances adipocyte differentiation and lipid accumulation.
    • Matrigel® provides an improved in vivo-like environment for studying adipogenesis due to increased cell-cell and cell-ECM interactions.
    • These findings highlight the value of Matrigel® for 3T3-L1 cell culture and adipogenesis research.