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Updated: Oct 27, 2025
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An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
Published on: December 3, 2020
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Microbial leakage evaluation of warm gutta - percha techniques
A Libonati1, V Di Taranto1, E Montemurro1
1Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", Rome, Italy.
Journal of Biological Regulators and Homeostatic Agents
|July 22, 2021
Summary
This study compared bacterial leakage in root canals using MicroHeat and continuous wave obturation techniques. Results showed no significant difference in leakage between techniques, regardless of sealer use, after 60 days.
Area of Science:
- Endodontics
- Dental Materials Science
Background:
- Bacterial leakage is a critical factor in endodontic treatment failure.
- Understanding obturation techniques' impact on sealing is crucial for long-term success.
Purpose of the Study:
- To compare bacterial leakage between MicroHeat and continuous wave obturation methods.
- To evaluate the influence of endodontic sealer on leakage in both techniques.
Main Methods:
- Thirty-eight mandibular premolars were prepared and obturated using MicroHeat or System B, with or without sealer.
- Samples were subjected to daily bacterial infiltration testing for 60 days.
- Statistical analysis was performed to compare contamination levels between groups.
Main Results:
- At 32 days, System B without sealer showed the lowest contamination (37.8%).
- After 60 days, contamination reached 100% in three groups (MicroHeat with sealer, MicroHeat without sealer, System B with sealer).
- No statistically significant differences in bacterial leakage were found between the groups at the end of the study.
Conclusions:
- Neither MicroHeat nor continuous wave obturation technique demonstrated superior sealing ability against bacterial leakage.
- The use of endodontic sealer did not significantly alter the long-term bacterial leakage outcomes in this study.
