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A 'smart' aptamer-functionalized continuous label-free cell catch-transport-release system.

Bozhen Zhang1, Canran Wang1, Yingjie Du1

  • 1Department of Materials Science and Engineering, University of California, Los Angeles, CA 90095, USA. ximinhe@ucla.edu.

Journal of Materials Chemistry. B
|July 22, 2021
PubMed
Summary

A novel hydrogel device (iCatch) enables continuous, selective, label-free cell sorting. This thermo-chemo-mechanical system improves cell separation for biomedical research and therapeutics.

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Area of Science:

  • Biomedical engineering
  • Materials science
  • Cell biology

Background:

  • Label-free cell sorting is crucial for research and therapeutics.
  • Existing methods lack simultaneous continuity and selectivity, leading to complexity and unreliability.

Purpose of the Study:

  • To develop an advanced label-free cell sorting device.
  • To overcome limitations of current cell separation technologies.

Main Methods:

  • Development of an immunoaffinity-based hydrogel (iCatch) device.
  • Integration of a temperature-responsive hydrogel with affinity handles for cell capture, transport, and release.
  • Utilizing a biphasic microfluidic platform for cell manipulation.
  • Validation using CCRF-CEM cells and aptamers.

Main Results:

  • The iCatch device demonstrated cell catching efficiency of approximately 40% and a recovery rate of about 70%.
  • Achieved high-throughput sorting at approximately 900 cells mm⁻¹ s⁻¹.
  • Validated biocompatibility and sorting capabilities.

Conclusions:

  • The iCatch device offers a continuous and selective platform for label-free cell sorting.
  • This technology has significant potential for downstream biomedical applications.
  • The thermo-chemo-mechanical coupling hydrogel system represents an advancement in cell separation technology.