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Updated: Oct 27, 2025

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
Published on: July 31, 2021
Discrimination of PHIP Signals Through their Evolution in Multipulse Sequences
S Bussandri1,2, M B Franzoni1,2, L Buljubasich1,2
1Universidad Nacional de Córdoba, Facultad de Matemática, Astronomía, Física y Computación, Córdoba, Argentina.
Abstract:
The antiphase character of the PHIP associated signals after a hydrogenation reaction is particularly sensitive to line broadening introduced by magnetic field inhomogeneities and interferences by the presence of resonance lines steaming from a large amount of thermally polarized spins. These obstacles impose a limitation in the detection of reaction products as well as in the experimental setups. A simple way to overcome these impediments consists of acquiring the signal with a train of refocusing pulses instead of a single r.f. pulse. We present here a number of examples where this multipulse acquisition, denominated PhD-PHIP, displays its potentiality in improving the information related to hyperpolarized spins performed in a sample, where the former parahydrogen nuclei are part of a complex J-coupling network.
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