Related Experiment Videos
Creatine kinase BB activity in serum of patients with acute stroke: correlation with the severity of brain damage
1Istituto Malattie Nervose e Mentali, Università degli Studi di Perugia.
Insights
Serum levels of creatine kinase BB (BB-CK) were significantly elevated in stroke patients compared to controls. Higher BB-CK concentrations correlated with greater brain damage severity in stroke survivors.
Area of Science:
- Biochemistry
- Neurology
- Clinical Diagnostics
Background:
- Stroke is a leading cause of long-term disability and mortality.
- Biomarkers for early and accurate stroke diagnosis and prognosis are crucial.
- Creatine kinase BB (BB-CK) is an isoenzyme predominantly found in the brain.
Purpose of the Study:
- To investigate the diagnostic potential of serum BB-CK levels in acute stroke.
- To explore the correlation between serum BB-CK concentrations and stroke severity.
Main Methods:
- Serum samples were collected from 11 acute stroke patients and 10 healthy controls approximately 36 hours post-stroke onset.
- BB-CK levels were quantified using ion-exchange column separation and gradient elution.
- Clinical data and CT scans were used to assess stroke severity.
Main Results:
- Mean serum BB-CK concentration was significantly higher in stroke patients than in controls (p < 0.01).
- A positive correlation was observed between serum BB-CK levels and the severity of brain damage in stroke patients.
Conclusions:
- Elevated serum BB-CK levels may serve as a potential biomarker for acute stroke.
- Serum BB-CK concentration shows promise in reflecting the extent of brain damage following a stroke.
Abstract:
BB-CK activity was measured in 11 patients with stroke and in 10 controls. Blood samples were taken 36 hours after the clinical stroke onset in every patient. Sera were stored at -80 degrees and analyzed within two months. The creatine kinase isoenzymatic pattern was determined by ion-exchange column separation and gradient elution system. The mean BB-CK concentration in patients with stroke was significantly higher than in controls (p less than 0.01). In the group of "stroke" patients we found a correlation between severity of brain damage, as suggested by the clinical picture and CT scans, and serum values of BB-CK.