miR-671-5p repressed progression of papillary thyroid carcinoma via TRIM14

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Dysregulated microRNAs (miRNAs) play a key role in papillary thyroid carcinoma (PTC) development.
• miR-671-5p has been identified as a potential tumor suppressor, but its precise role in PTC progression requires further investigation.

Purpose of the Study:

• To elucidate the role and underlying mechanism of miR-671-5p in papillary thyroid carcinoma progression.
• To investigate miR-671-5p's effect on cell viability, proliferation, migration, and invasion in PTC cells.
• To identify and validate the downstream target of miR-671-5p involved in PTC.

Main Methods:

• Functional assays were performed to assess the impact of miR-671-5p overexpression on PTC cell behavior.
• In vivo studies were conducted to evaluate the effect of miR-671-5p on tumor growth.
• Western blotting and luciferase reporter assays were used to identify and validate Tripartite Motif Containing 14 (TRIM14) as a direct target of miR-671-5p.

Main Results:

• Forced expression of miR-671-5p significantly decreased PTC cell viability, proliferation, migration, and invasion.
• In vivo, miR-671-5p overexpression inhibited tumor growth, reduced Ki67 expression, and decreased tumor volume and weight.
• TRIM14 was confirmed as a direct target of miR-671-5p, with its expression being suppressed by miR-671-5p. Overexpression of TRIM14 reversed the inhibitory effects of miR-671-5p.

Conclusions:

• miR-671-5p functions as a tumor suppressor in papillary thyroid carcinoma by inhibiting progression.
• The tumor-suppressive activity of miR-671-5p is mediated through the downregulation of its target gene, TRIM14.
• miR-671-5p-TRIM14 axis represents a promising therapeutic strategy for papillary thyroid carcinoma.