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Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

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Published on: April 21, 2016

From Thermogelling Hydrogels toward Functional Bioinks: Controlled Modification and Cytocompatible Crosslinking.

Lukas Hahn¹, Matthias Beudert², Marcus Gutmann²

¹Functional Polymer Materials, Chair for Advanced Materials Synthesis, Institute for Functional Materials and Biofabrication, Department of Chemistry and Pharmacy and Bavarian Polymer Institute, Julius-Maximilians-University Würzburg, Röntgenring 11, Würzburg, 97070, Germany.

Macromolecular Bioscience

|July 22, 2021

Summary

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This study introduces a novel bioink using Diels-Alder chemistry for stable, printable hydrogels. The resulting materials demonstrate excellent shape fidelity and biocompatibility for cell encapsulation in biofabrication.

Area of Science:

Biomaterials Science
Polymer Chemistry
Tissue Engineering

Background:

Hydrogels are crucial for biofabrication, providing printability and stability.
Diels-Alder chemistry offers a versatile platform for hydrogel modification.

Purpose of the Study:

To develop a novel bioink system based on thermogelling diblock copolymers.
To utilize Diels-Alder click chemistry for enhanced hydrogel stability and cell integration.

Main Methods:

Diblock copolymers of poly(2-methyl-2-oxazoline) and poly(2-n-propyl-2-oxazine) were synthesized and modified.
Post-polymerization modification involved hydrolysis and coupling with furan and maleimide moieties.
RGD-peptides were incorporated using thiol-maleimide chemistry for cell adhesion.

Keywords:

biofabrication bioprinting chemical crosslinking hydrogels

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Main Results:

The modified diblock copolymers exhibited excellent printability due to thermogelling and shear-thinning properties.
Trigger-less Diels-Alder click chemistry ensured long-term shape fidelity.
Functionalization with RGD-peptides promoted fibroblast adhesion, proliferation, and good cytocompatibility in encapsulated cells.

Conclusions:

A versatile two-step bioink system combining physical gelation and chemical crosslinking was established.
The platform allows for easy incorporation of bioactive moieties for enhanced cellular interaction and biomechanical properties.
The developed bioink demonstrates high potential for advanced biofabrication applications.