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Affinity monolith chromatography: A review of general principles and recent developments
Saumen Poddar1, Sadia Sharmeen1, David S Hage1
1Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
Electrophoresis
|July 22, 2021
Summary
Affinity monolith chromatography (AMC) uses specialized supports with binding agents for precise separation and detection of target compounds. This review covers AMC principles, materials, immobilization techniques, and diverse applications in analytical chemistry.
Area of Science:
- Analytical Chemistry
- Separation Science
- Biochemistry
Background:
- Affinity monolith chromatography (AMC) is a powerful liquid chromatography technique.
- It employs monolithic supports functionalized with specific binding agents.
- AMC enables the isolation, enrichment, and detection of target analytes within complex mixtures.
Purpose of the Study:
- To review the fundamental principles of Affinity Monolith Chromatography (AMC).
- To discuss recent advancements and developments in the field of AMC.
- To provide an overview of materials, immobilization strategies, and applications of AMC.
Main Methods:
- Discussion of various monolithic support materials: organic, inorganic, hybrid, carbohydrate, and cryogel.
- Examination of binding agent immobilization techniques: covalent immobilization, biospecific adsorption, entrapment, molecular imprinting, and coordination.
- Overview of recently employed binding agents and their integration into AMC systems.
Main Results:
- AMC offers target-specific interactions for effective separation and detection.
- Diverse applications include bioaffinity, immunoaffinity, immobilized metal-ion affinity, and dye-ligand chromatography.
- AMC is also valuable for chiral separations and biointeraction studies.
Conclusions:
- Affinity monolith chromatography is a versatile technique with broad applicability.
- Recent developments continue to expand its utility in various scientific domains.
- AMC provides efficient solutions for complex separation and detection challenges.
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