Effects of preparation parameters on the properties of the crosslinked pectin nanofiber mats

Pectin nanofiber mats prepared with periodate oxidation-electrospinning-adipic acid dihydrazide crosslinking strategy are promising for biomedical applications. In this study, we systematically examined the effects of electrospinning and crosslinking conditions on the properties of pectin nanofiber mats. The properties of mats were tunable in the range of 200-400 nm fiber size, 11-21% ADH residue content, 13-28 times absorbency, 13°-21° contact angle, 2 weeks or longer degradation time, 1.5-2.2 MPa tensile strength, 40-70% elongation, and 0.25-0.27 g/(cm²·24 h) permeability. Increasing polymer concentration, adipic acid dihydrazide amount, time or temperature could increase fiber size and its tensile strength, and decrease the absorbency, hydrophilicity, degradation rate, and elongation. These results indicate that controlling the process parameters can effectively regulate the properties of pectin nanofiber mats and meet the requirements of various biomedical applications.