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Characteristics and antifatigue activity of graded polysaccharides from Ganoderma lucidum separated by cascade
Ming Cai1, Haoyong Xing1, Baoming Tian1
1Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, People's Republic of China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, People's Republic of China.
Abstract:
In this paper, cascade membrane technology was utilized to classify polysaccharides from Ganoderma lucidum (GLPs). The properties and antifatigue activity of graded polysaccharides were identified and compared. GLPs were separated using cascade ultrafiltration membranes of 100 kDa, 10 kDa and 1 kDa in sequence. The molecular weights of polysaccharides in these GLP fractions were approximately 322.0 kDa, 18.8 kDa and 6.4 kDa, and all polysaccharides were in active β-configurations. This showed that all graded GLPs could elongate swimming time, improve endurance and promote fatigue recovery, especially polysaccharides with molecular weights above 10 kDa. This demonstrated that GLPs could decrease the activities of SUN and CK and the levels of MDA and BLA. They also increased the level of Gly, accelerated fat transformation, and improved the activities of GPx, SOD and LDH in all treated mice. Accordingly, GLPs above 10 kDa might be potential agents with antifatigue activity.
