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Genomic Sequence Analysis of Bombyx mori Nucleopolyhedrovirus Isolated from Yunnan Sericulture Region, China

Yonghong Zhang1, Feng Zhu2, Yulan Shao1

  • 1Sericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Science, Mengzi, Yunnan China.

Indian Journal of Microbiology
|July 23, 2021
PubMed

Insights

This study details the Bombyx mori nucleopolyhedrovirus (BmNPV) strain from Baoshan, Yunnan. Genetic analysis reveals unique gene deletions and structural variations, offering insights into BmNPV evolution and sericulture protection.

Area of Science:

  • Virology
  • Genomics
  • Entomology

Background:

  • Bombyx mori nucleopolyhedrovirus (BmNPV) causes grasserie, a significant disease impacting the sericulture industry.
  • Understanding the genetic diversity of endemic BmNPV strains is crucial for disease management and prevention.

Purpose of the Study:

  • To characterize the genome structure and sequence of a BmNPV isolate from Baoshan, Yunnan.
  • To compare the BmNPV-Baoshan strain with known BmNPV strains to understand its genetic status and evolutionary relationships.

Main Methods:

  • Isolation and sequencing of the BmNPV-Baoshan strain.
  • Genome assembly and analysis, including G+C content determination.
  • Phylogenetic analysis to determine evolutionary relationships with other BmNPV strains.

Main Results:

  • The BmNPV-Baoshan isolate possesses a genome of 128,452 bp with a 40.4% G+C content.
  • Phylogenetic analysis places BmNPV-Baoshan among Chinese BmNPV isolates, closely related to BomaNPV-S1.
  • The BmNPV-Baoshan strain exhibits a deletion in the bro-b gene and a reduction in repeat units within the hr1 gene compared to the BmNPV-T3 strain.

Conclusions:

  • The genetic characterization of BmNPV-Baoshan provides valuable data on the evolution of BmNPV strains in China.
  • Identifying genetic variations like gene deletions and structural changes aids in understanding viral adaptation and pathogenicity.
  • These findings contribute to strategies for protecting the sericulture industry from BmNPV-induced diseases.
Keywords:
BaculovirusBaoshan isolateBmNPV isolateBombyx moriComplete genome

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