Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

100
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
100
Defenses Against Pathogens and Herbivores02:26

Defenses Against Pathogens and Herbivores

28.1K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
28.1K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

1.7K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.7K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

167
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
167
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

133
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
133
Bacterial Signaling01:30

Bacterial Signaling

37.4K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
37.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

What Is the Functional Role of the Acyltransferase-like Domain in the Svx Peptidase of the Phytopathogenic Bacterium <i>Pectobacterium atrosepticum</i>?

International journal of molecular sciences·2026
Same author

Transfer learning in DeepLC improves LC retention time prediction across substantially different modifications and setups.

Nature communications·2026
Same author

Transcriptomic data sets for <i>Salmonella enterica</i> serovar Typhimurium 14028S that survived ingestion by <i>Acanthamoeba castellanii</i>, hydrogen peroxide treatment, or starvation.

Microbiology resource announcements·2025
Same author

Chemotherapy-induced neuropathy in monomethyl Auristatin E treatment: prevention by lithium.

British journal of cancer·2025
Same author

Cappable-seq RNA-sequencing data sets for comparative studying of <i>Salmonella enterica</i> adaptation to <i>Acanthamoeba castellanii</i> intracellular environment, oxidative stress, and starvation.

Microbiology resource announcements·2025
Same author

Transcriptome sequencing data sets of <i>Escherichia coli</i> K-12 MG1655 treated with novobiocin, tetracycline, and rifampicin.

Microbiology resource announcements·2025

Related Experiment Video

Updated: Oct 27, 2025

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

22.2K

Stringent Response in Bacteria and Plants with Infection.

Olga Petrova1, Olga Parfirova1, Yuri Gogolev1

  • 1Kazan Institute of Biochemistry and Biophysics, Federal Research Center, Kazan Scientific Center of Russian Academy of Sciences, Kazan 420111, Russian Federation.

Phytopathology
|July 23, 2021
PubMed
Summary

Stringent response (SR) in bacteria and plants is activated during infection. Plant responses vary, with potato showing resistance and tobacco exhibiting susceptibility, highlighting evolutionary interactions.

Keywords:
(p)ppGppRelA-SpoT homologsbacterial infectionhost plantjasmonic acid defense pathwaystringent response

More Related Videos

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection
07:25

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Published on: May 24, 2018

10.7K
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

9.2K

Related Experiment Videos

Last Updated: Oct 27, 2025

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

22.2K
Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection
07:25

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Published on: May 24, 2018

10.7K
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

9.2K

Area of Science:

  • Microbiology
  • Plant Science
  • Molecular Biology

Background:

  • Stringent response (SR) is a crucial stress response in bacteria and plant chloroplasts.
  • SR acts as a molecular switch, reprogramming transcription during stress.
  • Plant-microbial interactions involve stress for both host and pathogen.

Purpose of the Study:

  • To investigate the expression of bacterial and plastid SR-related genes during plant-microbial interactions.
  • To understand the differential responses of potato and tobacco plants to bacterial infection.

Main Methods:

  • Analysis of SpoT-dependent SR induction in *Pectobacterium atrosepticum* upon contact with host plants.
  • Examination of plastid SR and defense pathway activation (salicylic acid, jasmonic acid) in *Solanum tuberosum* and *Nicotiana tabacum*.

Main Results:

  • Bacterial SR was induced in *Pectobacterium atrosepticum* after interacting with potato and tobacco.
  • Potato plants (*Solanum tuberosum*), exhibiting long-term co-evolution, did not induce plastid SR but activated salicylic acid pathways for resistance.
  • Tobacco plants (*Nicotiana tabacum*), an experimental host, showed SR activation alongside jasmonic acid pathways, leading to plant death.

Conclusions:

  • Plant and bacterial SR gene expression differs during infection, influenced by evolutionary history.
  • Co-evolution shapes plant defense strategies, with SR playing a role in susceptibility or resistance.
  • Understanding these interactions is key to deciphering plant-pathogen evolutionary dynamics.