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Hypersensitivity response has negligible impact on Hematopoietic Stem Cells
Nir Bujanover1, Roshina Thapa1, Oron Goldstein2
1The Shraga Segal Department of Microbiology Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, 84105, Israel; National Institute for Biotechnology in the Negev, 84105, Israel.
Stem Cell Reports
|July 23, 2021
Summary
Hypersensitivity immune responses do not significantly alter hematopoietic stem cells (HSCs) in the bone marrow. This study found no changes in HSC markers, potency, or proliferation, contrasting with other immune stimuli.
Area of Science:
- Immunology
- Hematology
- Stem Cell Biology
Background:
- Immune cells originate from hematopoietic stem cells (HSCs) in the bone marrow (BM).
- Immune stimulation typically activates quiescent HSCs to rapidly generate immune cells.
- HSCs respond to various viral and bacterial stimuli.
Purpose of the Study:
- To investigate the impact of hypersensitivity immune response on HSCs.
- To determine if hypersensitivity alters HSC activation, proliferation, or function.
- To compare the effect of hypersensitivity on HSCs with other immune stimuli.
Main Methods:
- Utilized an Ova-induced hypersensitivity peritonitis model in mice.
- Analyzed HSC populations in the bone marrow using flow cytometry for key markers (cKIT, SCA1, CD48, CD150, Fgd5-mCherry).
- Assessed HSC function through transplantation assays.
- Performed RNA-sequencing on HSCs to analyze transcriptomic changes.
- Evaluated HSC proliferation status.
Main Results:
- No significant differences were observed in HSC markers between hypersensitivity and control groups.
- Hypersensitivity did not alter the functional potency of HSCs as determined by transplantation.
- RNA-sequencing revealed only minor changes at the transcriptome level.
- No significant change in the proliferative state of HSCs was detected.
Conclusions:
- Hypersensitivity immune responses, unlike other immune stimuli, do not significantly impact bone marrow hematopoietic stem cells.
- HSCs remain unaffected in terms of number, markers, function, and proliferation during hypersensitivity.
- This finding contrasts with the known activation of HSCs by other immune challenges.
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