Related Experiment Video
Updated: Oct 27, 2025
13:56
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Published on: July 18, 2013
11.4K
Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression
Gislaine Curty1, Albert N Menezes2, Ayslan C Brant3
1Oncovirology Program, Instituto Nacional de Câncer (INCA), Rio de Janeiro 20231-050, Brazil.
Cancers
|July 24, 2021
Summary
Retroelements, including human endogenous retroviruses (HERVs) and long interspersed nuclear elements (L1s), are expressed in cervical cancer. Their expression varies with HPV infection and influences neighboring gene activity.
Area of Science:
- Oncology
- Genomics
- Virology
Background:
- Retroelements are implicated in cancer development and progression.
- Their role in cervical cancer, particularly in relation to HPV, requires further elucidation.
Purpose of the Study:
- To characterize retroelement expression in cervical cancer.
- To investigate the interplay between retroelements, HPV infection, and host gene expression.
Main Methods:
- Analysis of RNA-seq data from 40 cervical carcinoma biopsies.
- Genotyping of HPV and quantification of human endogenous retrovirus (HERV) and long interspersed nuclear element 1 (L1) expression.
- Correlation analysis between retroelement expression, HPV types, and neighboring gene expression.
Main Results:
- Over 8000 retroelements were expressed in cervical cancer samples.
- 103 retroelements showed differential expression based on tumor histology and HPV type.
- Specific HERV and L1 elements were associated with HPV status and influenced expression of nearby genes like IL20RB.
Conclusions:
- Retroelement expression is a feature of cervical cancer.
- HPV status significantly impacts the differential expression of L1 elements.
- Retroelement location can modulate the expression of adjacent host genes, suggesting a role in cervical carcinogenesis.
More Related Videos
10:26RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
27.7K
13:19Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
Published on: November 2, 2013
16.8K
Related Concept Videos
01:51Mechanisms of Retrovirus-induced Cancers
5.4K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.4K
03:18Non-LTR Retrotransposons
12.2K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.2K
01:03Rous Sarcoma Virus (RSV) and Cancer
5.6K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.6K
02:33Retroviruses
13.2K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
13.2K
01:10Retrovirus Life Cycles
47.8K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
47.8K
03:08LTR Retrotransposons
18.3K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
18.3K