Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wavy geometry controls nuclear morphology, migration, and YAP signaling independent of myosin II contractility.

Acta biomaterialia·2026
Same author

A GelMA/polydopamine hydrogel with PTH and osteogenically stimulated alveolar mucosa-derived stem cells promotes bone regeneration in MRONJ-affected wounds.

Stem cell research & therapy·2025
Same author

Tension anisotropy drives fibroblast phenotypic transition by self-reinforcing cell-extracellular matrix mechanical feedback.

Nature materials·2025
Same author

Deep learning for the identification of ridge deficiency around dental implants.

Clinical implant dentistry and related research·2023
Same author

Alveolar mucosal cell spheroids promote extraction socket healing and osseous defect regeneration.

Journal of periodontology·2023
Same author

Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects.

Stem cell research & therapy·2023

Related Experiment Video

Updated: Sep 25, 2025

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

2.4K

3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction.

Hsu-Hsiang Lin1, Pen-Hsiu Grace Chao2, Wei-Chiu Tai3

  • 1Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei 10048, Taiwan.

International Journal of Molecular Sciences
|July 24, 2021
PubMed
Summary

This study developed 3D-printed collagen waveform microfibers to regenerate the periodontal ligament (PDL). These scaffolds promote cell viability and healing under shear stress, offering a promising solution for PDL regeneration.

Keywords:
bioprintingcollagenperiodontal ligamenttissue engineering

More Related Videos

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds
06:36

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds

Published on: April 24, 2019

9.7K
Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels
12:07

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels

Published on: February 12, 2016

9.3K

Related Experiment Videos

Last Updated: Sep 25, 2025

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

2.4K
3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds
06:36

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds

Published on: April 24, 2019

9.7K
Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels
12:07

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels

Published on: February 12, 2016

9.3K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Periodontal ligament (PDL) regeneration is challenging.
  • Functional requirements for PDL tissue are difficult to meet.
  • A biomimetic system is needed to support PDL regeneration.

Purpose of the Study:

  • To develop a biomimetic microfibrous system for PDL regeneration.
  • To investigate the effect of fluidic shear stress on PDL cells seeded on microfibers.
  • To assess the potential of collagen-based microfibers in promoting PDL healing.

Main Methods:

  • Fabrication of collagen-based straight and waveform microfibers using extrusion-based bioprinting.
  • Application of laminar flow-based bioreactor to generate fluidic shear stress (0 or 6 dynes/cm²).
  • Assessment of PDL cell viability, morphology, adhesion, and gene expression.

Main Results:

  • Successfully fabricated collagen-based microfibers with optimized bioprinting.
  • Waveform microfibers were wider (235.9 ± 11.22 μm) than straight microfibers (189.9 ± 11.44 μm).
  • PDL cells showed enhanced viability, adhesion, and cytoskeleton expansion under 6 dynes/cm² shear stress.
  • Upregulation of Cyclin D, E-cadherin, and periostin observed on waveform microfibers.

Conclusions:

  • 3D-printed collagen-based waveform microfibers maintain PDL cell viability.
  • Waveform microfibers show enhanced potential for promoting healing and regeneration under shear stress.
  • This approach offers a promising guiding scaffold for periodontal ligament regeneration.