Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe

Area of Science:

• Biochemistry
• Chemical Biology
• Molecular Imaging

Background:

• Nitric oxide (NO) is a critical signaling molecule in numerous physiological and pathological processes.
• Accurate monitoring of NO levels is essential for biological and biochemical research.
• Fluorescent imaging offers a powerful method for visualizing NO concentration in situ.

Purpose of the Study:

• To design and synthesize a novel small-molecule fluorescent probe for sensitive and selective nitric oxide detection.
• To evaluate the probe's performance in aqueous media and biological environments.
• To demonstrate the probe's utility for nitric oxide sensing in living cells.

Main Methods:

• Synthesis of a novel 9(10H)acridone-based fluorescent probe.
• Investigation of the probe's reaction with nitric oxide in aqueous solution under aerobic conditions.
• Spectroscopic analysis to quantify changes in fluorescence intensity upon NO interaction.
• Cellular imaging experiments using Jurkat cells to assess probe performance in a biological context.

Main Results:

• The developed 9(10H)acridone derivative exhibits a fivefold increase in fluorescence intensity upon reaction with nitric oxide.
• The probe demonstrates effective nitric oxide sensing in aqueous media in the presence of oxygen.
• Successful application of the probe for detecting nitric oxide in living Jurkat cells was achieved.

Conclusions:

• A novel and effective fluorescent probe for nitric oxide has been successfully developed.
• The probe offers enhanced fluorescence signaling upon NO detection, suitable for biological applications.
• This tool facilitates real-time monitoring of nitric oxide in living cells, advancing biological and biochemical studies.