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Updated: Oct 27, 2025
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines using differential
Laura Bryan1, Michael Henry2, Ronan M Kelly3
1National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. laura.bryan2@mail.dcu.ie.
Background:
The ability to achieve high peak viable cell density earlier in CHO cell culture and maintain an extended cell viability throughout the production process is highly desirable to increase recombinant protein yields, reduce host cell impurities for downstream processing and reduce the cost of goods. In this study we implemented label-free LC-MS/MS proteomic profiling of IgG4 producing CHO cell lines throughout the duration of the cell culture to identify differentially expressed (DE) proteins and intracellular pathways associated with the high peak viable cell density (VCD) and extended culture VCD phenotypes.
Results:
We identified key pathways in DNA replication, mitotic cell cycle and evasion of p53 mediated apoptosis in high peak VCD clonally derived cell lines (CDCLs). ER to Golgi vesicle mediated transport was found to be highly expressed in extended culture VCD CDCLs while networks involving endocytosis and oxidative stress response were significantly downregulated.
Conclusion:
This investigation highlights key pathways for targeted engineering to generate desirable CHO cell phenotypes for biotherapeutic production.
