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Published on: March 2, 2016
Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational
Robert Ruemmler1, Alexander Ziebart2, Elisabeth Britten2
1Department of Anesthesiology, Medical Centre of the Johannes Gutenberg University, Langenbeckstrasse 1, 55131, Mainz, Germany. Robert.ruemmler@email.de.
Objective:
Extracellular histones have been identified as one molecular factor that can cause and sustain alveolar damage and were linked to high mortality rates in critically ill patients. In this pilot study, we wanted to validate the proinflammatory in vivo effects of local histone application in a prospective translational porcine model. This was combined with the evaluation of an experimental acute lung injury model using intrabronchial lipopolysaccharides, which has been published previously.
Results:
The targeted application of histones was successful in all animals. Animals showed decreased oxygenation after instillation, but no differences could be detected between the sham and histone treatments. The histologic analyses and inflammatory responses indicated that there were no differences in tissue damage between the groups.
