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Updated: Oct 27, 2025

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis
Published on: March 28, 2025
Personalized profiles of antioxidant signaling pathway in patients with tuberculosis
Sara Petrillo1, Maria Giovanna Gallo1, Annapaola Santoro2
1Unit of Neuromuscular and Neurodegenerative Diseases, Department of Neuroscience, Children's Hospital Bambino Gesù, IRCCS, Rome, Italy.
Background/Purpose:
The non-protein thiol glutathione is protective against infection by Mycobacterium tuberculosis (MTB) and, together with the transcription factor NRF2 (the nuclear factor erythroid 2-related factor 2), plays a crucial role in counteracting MTB-induced redox imbalance. Many genes implicated in the antioxidant response belong to the NRF2-signalling pathway, whose central role in the pathogenesis of tuberculosis (TB) has been recently proposed.
Methods:
In this study, we measured GSH levels in blood of patients with active TB and analysed the individual NRF2-mediated redox profile, in order to provide additional tools for discriminating the pathologic TB state and addressing therapeutic interventions.
Results:
Our findings show a systemic individual modulation of GSH and NRF2 signaling pathway in patients with TB, with a "personalized" induction of NRF2-target genes.
Conclusion:
This study can provide useful tools to monitor the course of the infection and address patients' treatment.
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