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Methylation and expression levels of microRNA-23b/-24-1/-27b, microRNA-30c-1/-30e, microRNA-301a and let-7g are

I Gilyazova1,2, E Ivanova3, G Gilyazova2

  • 1Institute of Biochemistry and Genetics - Subdivision, Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa, Russian Federation, 450054.

Molecular Biology Reports
|July 24, 2021
PubMed
Abstract

Insights

MicroRNA gene methylation changes, specifically involving microRNA-301a, microRNA-23b, and let-7g, are associated with clear cell renal cell carcinoma development. Further research is needed to confirm their role in kidney cancer pathogenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Renal cell carcinoma (RCC) is the predominant kidney cancer in adults.
  • DNA methylation and microRNA regulation are key in controlling gene activity.
  • Dysregulation of these mechanisms can lead to cellular signaling pathway impairment, as seen in cancers.

Purpose of the Study:

  • To investigate the potential association between microRNA gene methylation and renal cancer.
  • To explore the methylation status and expression levels of specific microRNAs in clear cell renal cell carcinoma (ccRCC).

Main Methods:

  • Analyzed methylation levels of 22 microRNA genes in tumor and normal kidney tissues from 30 ccRCC patients (TNM Stage III).
  • Utilized a pathway-specific real-time polymerase chain reaction array for methylation analysis.
  • Performed quantitative polymerase chain reaction for microRNA expression profiling.

Main Results:

  • Identified significant methylation differences in specific microRNA genes and clusters.
  • Observed hypermethylation of microRNA-23b/-24-1/-27b, microRNA-30c-1/-30e, and let-7g in ccRCC tissues.
  • Found hypomethylation of microRNA-301a in tumor tissues compared to normal tissues.
  • Detected significant overexpression of microRNA-301a and microRNA-23b, and downregulation of let-7g in ccRCC.

Conclusions:

  • Results suggest a potential role for microRNA-301a, microRNA-23b, and let-7g in the pathogenesis of renal cancer.
  • Further studies are required to elucidate the functional significance of these observed epigenetic and expression changes in ccRCC.
Keywords:
DNA methylationRenal cell carcinomamicroRNA

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