LncRNA SNHG20 promotes cell proliferation and invasion by suppressing miR-217 in ovarian cancer
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Published on: August 2, 2024
Xuefeng Xing1, Ming An2, Tonghua Chen1
1Sanya People's Hospital, Sanya, Hainan, China.
Background:
Ovarian cancer is the most common female gynecological malignancy. SNHG20, as a long non-coding RNA, has been proven to be an important regulator in the occurrence and development of various tumors. However, the potential mechanism of SNHG20 in ovarian cancer is unclear.
Objective:
The present study was aimed to investigate the functions and mechanisms of SNHG20 in ovarian cancer.
Methods:
The expression of SNHG20 and miR-217 in ovarian cancer tissues and cell lines was detected by qRT-PCR. CCK-8 assay was used to measure cell proliferation in transfected cells. The transwell assay was used to detect the relative invasion rate of transfected cells. The putative binding sites between SNHG20 and miR-217 were predicted by software LncBase v.2, and the interaction between SNHG20 and miR-217 was confirmed by dual-luciferase reporter assays and RIP assay. The rescue experiments were used to illustrate potential mechanisms.
Results:
SNHG20 was upregulated in ovarian cancer tissues and cell lines. Overexpression of SNHG20 promoted ovarian cancer cell proliferation and invasion. MiR-217 was downregulated in ovarian cancer tissues and cells, and was negatively regulated by SNHG20. Moreover, miR-217 overexpression inhibited ovarian cancer cell proliferation and invasion. Furthermore, miR-217 mimic reversed the inhibitory effect of SNHG20 overexpression on the biological behavior of ovarian cancer cells.
Conclusions:
SNHG20 promoted cell proliferation and invasion by sponging miR-217 in ovarian cancer. These results suggested that SNHG20 and miR-217 might provide new targets for therapeutic application in ovarian cancer.
Insights
Long non-coding RNA SNHG20 promotes ovarian cancer progression by sponging miR-217. This study reveals SNHG20 and miR-217 as potential therapeutic targets for ovarian cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Ovarian cancer is a leading gynecological malignancy.
- Long non-coding RNAs (lncRNAs) like SNHG20 regulate tumor development.
- The specific role of SNHG20 in ovarian cancer remains largely unknown.
Purpose of the Study:
- To investigate the functional role of SNHG20 in ovarian cancer.
- To elucidate the underlying molecular mechanisms of SNHG20 action.
- To explore the relationship between SNHG20 and miR-217 in ovarian cancer.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to assess SNHG20 and miR-217 expression.
- Cell Counting Kit-8 (CCK-8) and Transwell assays for proliferation and invasion.
- Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays to confirm interactions.
Main Results:
- SNHG20 expression was significantly upregulated in ovarian cancer tissues and cell lines.
- Overexpression of SNHG20 enhanced ovarian cancer cell proliferation and invasion.
- SNHG20 negatively regulated miR-217, which was downregulated in ovarian cancer.
- MiR-217 overexpression inhibited cell proliferation and invasion, reversing SNHG20's effects.
Conclusions:
- SNHG20 promotes ovarian cancer cell proliferation and invasion by acting as a molecular sponge for miR-217.
- SNHG20 and miR-217 represent promising novel therapeutic targets for ovarian cancer.
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