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Updated: Oct 27, 2025

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart
Published on: February 3, 2023
Single-Cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart
Manami Katoh1, Seitaro Nomura1,2, Shintaro Yamada1,2
1Genome Science Division, Research Center for Advance Science and Technology, The University of Tokyo, Tokyo, Japan.
Abstract:
Heart failure is caused by a complicated pathogenic process and has a poor prognosis. Quality of life is often impaired due to repeated hospitalization. Integrative analysis of the morphological, physiological, and molecular profiles of cardiomyocytes, which are responsible mainly for heart contraction, may lead to a deeper understanding of the pathogenesis of heart failure. However, unlike other types of cells, cardiomyocytes are relatively large, vulnerable to stress, and difficult to use for single-cell analysis. With some ingenuity, we have established a single-cardiomyocyte analysis pipeline. Here, we describe the procedure for single-cell RNA sequencing of adult mouse cardiomyocytes from isolation to analysis.
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