Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Facilitated Diffusion01:16

Facilitated Diffusion

787
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
787
The Significance of Membrane Transport01:44

The Significance of Membrane Transport

35.5K
The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
35.5K
Insertion of Multi-pass Transmembrane Proteins in the RER01:29

Insertion of Multi-pass Transmembrane Proteins in the RER

14.7K
The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...
14.7K
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

5.8K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
5.8K
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

8.3K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
8.3K
Membrane Fluidity01:26

Membrane Fluidity

13.3K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
13.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Shape of Things to Come: α-Helical Membrane Protein Folding on the Ribosome.

Chemical reviews·2026
Same author

Mg<sup>2+</sup>-dependent mechanism of environmental versatility in a multidrug efflux pump.

Structure (London, England : 1993)·2025
Same author

Mg<sup>2+</sup>-dependent mechanism of environmental versatility in a multidrug efflux pump.

bioRxiv : the preprint server for biology·2024
Same author

Editorial: Biophysics of co-translational protein folding.

Frontiers in molecular biosciences·2022
Same author

Methods to study folding of alpha-helical membrane proteins in lipids.

Open biology·2022
Same author

How lipids affect the energetics of co-translational alpha helical membrane protein folding.

Biochemical Society transactions·2022

Related Experiment Video

Updated: Oct 27, 2025

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
10:11

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

3.9K

Integrating Membrane Transporter Proteins into Droplet Interface Bilayers.

Heather E Findlay1, Nicola J Harris1, Paula J Booth2

  • 1Department of Chemistry, Kings College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|July 24, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed methods to incorporate membrane transporters into droplet interface bilayers (DIBs) for artificial cell applications. This enables controlled uphill transport of molecules, a key step for synthetic biology.

Keywords:
Cell-free expressionDroplet interface bilayerFluorescence microscopyMembrane proteinTransporter

More Related Videos

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
09:54

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

11.0K
Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.5K

Related Experiment Videos

Last Updated: Oct 27, 2025

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
10:11

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

3.9K
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
09:54

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

11.0K
Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.5K

Area of Science:

  • Synthetic biology
  • Biophysics
  • Biochemistry

Background:

  • Droplet interface bilayers (DIBs) are advanced tools for creating artificial cells and studying biological processes.
  • Controlled transport across membranes, especially uphill transport against concentration gradients, is crucial for DIB functionality.
  • Existing methods allow protein synthesis and DIB incorporation separately, but not combined for functional membrane transporters.

Purpose of the Study:

  • To present novel methods for incorporating membrane transporters into DIBs.
  • To demonstrate specific and uphill transport using these engineered DIBs.
  • To advance the development of artificial cells with controlled molecular transport.

Main Methods:

  • Developed two distinct methods for synthesizing and incorporating membrane transporters into DIBs.
  • Utilized a simple two-droplet DIB system for experimental setup.
  • Employed fluorescence microscopy to monitor transport reactions.

Main Results:

  • Successfully incorporated functional membrane transporters into DIBs.
  • Demonstrated both downhill and uphill transport of specific substrates.
  • Verified the ability of DIBs to facilitate transport against a concentration gradient.

Conclusions:

  • The presented methods enable the creation of DIBs with specific, active membrane transporters.
  • This work is a significant step towards building functional artificial cells.
  • The developed system allows for the study of uphill transport in a controlled environment.