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Updated: Oct 27, 2025

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
Published on: January 7, 2022
Plant mitochondria import DNA via alternative membrane complexes involving various VDAC isoforms
Tatiana A Tarasenko1, Ekaterina S Klimenko1, Vladislav I Tarasenko1
1Siberian Institute of Plant Physiology and Biochemistry, SB RAS, 132 Lermontov St, Irkutsk 664033, Russia.
Abstract:
Mitochondria possess transport mechanisms for import of RNA and DNA. Based on import into isolated Solanum tuberosum mitochondria in the presence of competitors, inhibitors or effectors, we show that DNA fragments of different size classes are taken up into plant organelles through distinct channels. Alternative channels can also be activated according to the amount of DNA substrate of a given size class. Analyses of Arabidopsis thaliana knockout lines pointed out a differential involvement of individual voltage-dependent anion channel (VDAC) isoforms in the formation of alternative channels. We propose several outer and inner membrane proteins as VDAC partners in these pathways.
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