Physiological responses of Pichia stipitis to imidazolium chloride ionic liquids with different carbon chain length

Ionic liquids (ILs) are used as detoxication agents for fermentation of lignin into ethanol because of their good applicability. However, the residual ILs may be toxic to the yeast. In order to improve the use of ILs for fermentation and protected environment, the toxicity of ILs with different carbon chain length to Pichia stipitis was studied in this paper. Four kinds of common imidazolium chloride ILs ([C₄mim]Cl, [C₆mim]Cl, [C₈mim]Cl and [C₁₀mim]Cl) were selected. ILs can inhibit the proliferation of Pichia stipitis and increase their mortality. Oxidative stress reaction occurred in the cells, and the activities of antioxidant enzymes are affected. Comparing with the integrated biomarker response (IBR) index, it was found that the toxicity increases with increasing chain length. ILs may enter cells by damaging cell membranes and reduce ethanol production by damaging organelles such as mitochondria. ILs caused wrinkles and dents on the surface of cells up to cell deformation and even rupture. The toxicity sequence was as follows: [C₁₀mim]Cl> [C₈mim]Cl>[C₆mim]Cl>[C₄mim]Cl. Due to this toxicity to Pichia stipitis, these compounds should be used carefully in the fermentation process and also to avoid toxic effects on other organisms in the environment.