Inhibitory effect of oil and fat on denitrification using food waste fermentation liquid as carbon source

Food waste fermentation liquid (FWFL) can be used as carbon source to enhance nitrogen removal in wastewater treatment. However, the influence of lipid, a common component of food waste, on denitrification remains unclear. In this study, the effect of oil and fat on denitrification process and the underlying mechanisms were investigated using synthetic oil- and fat-bearing carbon source and verified with real FWFL. In the batch experiment, oil and fat had no obvious influence on denitrification, but in the semi-continuous experiment, the denitrification rate in the oil- and fat-added assays decreased to 44% and 38% of that in the control, respectively, after 45 batches. Oil and fat caused sludge floatation, and the floating sludge thickness increased with the continuous operation. Oil/fat-sludge aggregates were observed in the floating sludge and limited gas release. Microbial community analysis indicated that oil and fat did not affect denitrifying bacteria abundance. Limitation of mass transfer might be the main reason for the inhibition of oil and fat on denitrification. In the real FWFL experiment, the denitrification rate in the original and emulsified oil-bearing FWFL decreased to 24% and 56% of that in the demulsifying FWFL, respectively, after 45 batches. These findings indicate the necessity of removing lipids when FWFL is used as denitrification carbon source.