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Rifampicin has - Compared to clindamycin - A dose and time dependent effect on hMSCs during osteogenic
Hannes Kubo1, Sarah Czerwinski1, Holger Schrumpf1
1University of Duesseldorf, Medical Faculty, Department of Orthopaedics and Trauma Surgery, Clinic Director: Prof. Joachim Windolf, Moorenstr. 5, D-40225, Duesseldorf, Germany.
Background:
The effects of rifampicin and clindamycin on human mesenchymal stromal cell (hMSC) were examined.
Methods:
hMSC were cultured with rifampicin and clindamycin (0.5 μg/ml, 5 μg/ml, 50 μg/ml) and examinations of proliferation (Bromodeoxyuridine), calcification (Alizarin red) and mineralization (alkaline phosphatase) were performed after 7th, 14th and 21st days.
Results:
With rifampicin (50 μg/ml) cultured hMSC showed a significant negative effect during proliferation, mineralization (7, 14 and 21 days) and calcification (21 days). Clindamycin seems to have no effect.
Conclusions:
Rifampicin in a dosage of 50 μg/ml showed a negative impact on proliferation, mineralization and calcification of hMSC after 21 days.
