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The Biological Function of TUSC7/miR-1224-3p Axis in Triple-Negative Breast Cancer

Bo-Hao Zheng1,2, Zhi-Xian He3, Juan Zhang4

  • 1Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, People's Republic of China.

Cancer Management and Research
|July 26, 2021
PubMed
Abstract

Insights

Low expression of long noncoding RNA TUSC7 is linked to poor survival in triple-negative breast cancer (TNBC). TUSC7 may act as a tumor suppressor by influencing cell growth, metastasis, and chemotherapy sensitivity.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple-negative breast cancer (TNBC) presents a significant clinical challenge due to its poor prognosis and lack of targeted therapies.
  • Long noncoding RNA (lncRNA) TUSC7, previously identified as a tumor suppressor in other cancers, warrants investigation in breast cancer.

Purpose of the Study:

  • To investigate the clinical significance of lncRNA TUSC7 in breast cancer.
  • To elucidate the biological function and underlying molecular mechanisms of TUSC7 in TNBC.

Main Methods:

  • Retrospective analysis of TUSC7 expression in 90 paired breast cancer tissues and adjacent normal tissues.
  • In vitro and in vivo assays to assess the impact of TUSC7 on tumor cell proliferation, migration, and invasion.
  • Mechanistic studies including microarray, luciferase reporter assays, and qPCR to explore TUSC7's regulatory pathways.

Main Results:

  • Low TUSC7 expression is an independent prognostic factor for poor overall survival in TNBC patients.
  • Overexpression of TUSC7 inhibited tumor growth in vitro and in vivo and enhanced sensitivity to paclitaxel and carboplatin.
  • TUSC7 interacts with miR-1224-3p and influences cell cycle, MAPK, PI3K/AKT, and NF-κB signaling pathways.

Conclusions:

  • Reduced TUSC7 expression serves as a prognostic marker for adverse outcomes in TNBC.
  • TUSC7 exhibits tumor-suppressive functions in breast cancer, potentially through modulation of miR-1224-3p and key signaling pathways.
Keywords:
MiR-1224-3PTUSC7long non-coding RNAprognosistriple-negative breast cancer

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