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Related Concept Videos

RNA Interference01:23

RNA Interference

26.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.8K
Experimental RNAi02:15

Experimental RNAi

6.6K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.6K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Types of RNA01:23

Types of RNA

69.8K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
69.8K
Types of RNA01:20

Types of RNA

7.1K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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RNA and DNA nanoparticles for triggering RNA interference.

Ziad El Tannir1, Kirill A Afonin1,2, Bruce A Shapiro2

  • 1Department of Chemistry, University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, North Carolina 28223, USA.

RNA & Disease (Houston, Tex.)
|July 26, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed novel nanoparticles for simultaneous activation of multiple RNA interference pathways and cellular functions. This platform offers potential for treating various diseases by precisely controlling gene silencing and other cellular processes.

Keywords:
FRETRNA interferenceRNA nanotechnologyRNA/DNA hybridssiRNAs

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanomedicine

Background:

  • Controlling in vivo delivery and synchronized activation of multiple cellular functions is complex.
  • Existing methods lack the precision for simultaneous multi-functional activation within cells.

Purpose of the Study:

  • To create a versatile platform for simultaneous activation of multiple RNA interference (RNAi) pathways and other cellular functionalities.
  • To engineer novel nanoparticle systems capable of precise, coordinated in vivo action.

Main Methods:

  • Design and synthesis of RNA, RNA/DNA, and DNA/RNA nanoparticles.
  • In vitro and in vivo testing of nanoparticle functionality and activation.
  • Evaluation of simultaneous RNAi pathway engagement and other functional delivery.

Main Results:

  • Successfully developed RNA, RNA/DNA, and DNA/RNA nanoparticles.
  • Demonstrated the capability of these nanoparticles for simultaneous activation of multiple cellular functions.
  • Confirmed successful engagement of multiple RNA interference pathways.

Conclusions:

  • The developed nanoparticle platform enables precise control over simultaneous activation of multiple cellular functionalities.
  • These novel nanostructures hold significant potential for targeted therapeutic applications across various diseases.