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Updated: Oct 26, 2025

Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment
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Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment

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F4, a collagen XIX-derived peptide, inhibits tumor angiogenesis through αvβ3 and α5β1 integrin interaction

Jean-Baptiste Oudart1,2, Matthieu Villemin1, Bertrand Brassart1

  • 1UMR CNRS/URCA 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne (URCA), Reims, France.

Cell Adhesion & Migration
|July 26, 2021
PubMed

Insights

The F4 peptide, derived from collagen XIX, inhibits melanoma cell migration and angiogenesis. It targets αvβ3 and α5β1 integrins, showing potential as an anti-cancer agent.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • F4 peptide from collagen XIX previously showed inhibition of melanoma cell migration and cancer progression.
  • The anti-angiogenic properties of F4 peptide were not previously investigated.

Purpose of the Study:

  • To investigate the anti-angiogenic properties of F4 peptide.
  • To identify the molecular targets of F4 peptide in endothelial cells.

Main Methods:

  • VEGF-induced pseudo-tube formation assay on Matrigel.
  • Rat aortic ring assay for endothelial sprouting.
  • Affinity chromatography and solid-phase assays to identify and confirm F4 peptide-integrin interactions.

Main Results:

  • F4 peptide inhibited VEGF-induced endothelial cell pseudo-tube formation and endothelial sprouting.
  • αvβ3 and α5β1 integrins were identified as potential receptors for F4 peptide.
  • Direct interaction between F4 peptide and both αvβ3 and α5β1 integrins was confirmed.

Conclusions:

  • F4 peptide exhibits potent anti-angiogenic properties.
  • F4 peptide directly interacts with αvβ3 and α5β1 integrins on endothelial cells.
  • F4 peptide is a promising antitumor agent due to its dual action on angiogenesis and tumor cell migration.
Keywords:
Extracellular matrixangiogenesiscollagen XIXintegrinmatrikine

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