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Human circular RNA hsa_circRNA_101705 (circTXNDC11) regulates renal cancer progression by regulating MAPK/ERK pathway

Chong-Yi Yang1, Jie Wang1, Jian-Qiu Zhang1

  • 1Department of Urology, Ninghai First Hospital, Zhejiang, China.

Bioengineered
|July 26, 2021
PubMed

Insights

Circular RNAs (circRNAs) are crucial in renal cell carcinoma (RCC) progression. This study reveals circTXNDC11 promotes RCC growth by activating the MAPK/ERK pathway, presenting a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in human tumor development.
  • Renal cell carcinoma (RCC) is a significant malignancy where circRNAs may influence progression.

Purpose of the Study:

  • To investigate the functional role and underlying mechanisms of human circular RNA hsa_circRNA_101705 (circTXNDC11) in RCC.
  • To determine if circTXNDC11 can serve as a potential therapeutic target for RCC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for expression analysis.
  • In vitro assays (CCK-8, colony formation, transwell) for proliferation and invasion.
  • Western blotting for protein analysis (MEK, ERK) and in vivo xenograft models.

Main Results:

  • circTXNDC11 was found to be overexpressed in RCC tissues and cell lines.
  • Overexpression of circTXNDC11 correlated with advanced TNM stage and lymph node metastasis.
  • Knockdown of circTXNDC11 inhibited RCC cell proliferation and invasion in vitro and reduced tumor growth in vivo.
  • circTXNDC11 promotes RCC progression by activating the MAPK/ERK signaling pathway.

Conclusions:

  • circTXNDC11 acts as a novel oncogenic regulator in RCC tumorigenesis.
  • circTXNDC11's promotion of RCC growth and invasion is mediated through the MAPK/ERK pathway.
  • circTXNDC11 represents a promising therapeutic target for renal cell carcinoma treatment.
Keywords:
Circular RNAsMAPK/ERKcircTXNDC11renal cancer

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