Logic-Gated Proximity Aptasensing for Cell-Surface Real-Time Monitoring of Apoptosis
Jiao Zheng1, Qiwei Wang1, Lin Shi1
1Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui, 230026, China.
Abstract:
In nature, intact apoptotic cells release ATP as a signaling molecule to trigger prompt phagocytic clearance, even at the earliest stage of apoptosis. Inspired by this, here we introduce a straightforward strategy for real-time monitoring ATP exocytosis and drug-stimulated apoptosis in the cancer cell surroundings. Triplex-boosted G-quadruplexes (tb-G4s) responding to cell environmental factors (H+ and K+ ) are engineered to construct a DNA logic-gated nanoplatform for proximity ATP aptasensing on the cell surface. It enables the real-time monitoring of cell apoptosis by capturing released endogenous ATP during chemotherapy drug stimulation, providing a sensitive approach for dynamically evaluating drug-induced apoptosis and therapeutic efficacy.
Insights
This study presents a DNA nanoplatform for real-time monitoring of ATP release and drug-induced apoptosis in cancer cells. It captures released ATP to dynamically evaluate chemotherapy efficacy.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Nanotechnology
Background:
- Apoptotic cells release ATP to signal for clearance.
- Monitoring ATP exocytosis and apoptosis is crucial for cancer therapy evaluation.
Purpose of the Study:
- To develop a real-time method for monitoring ATP exocytosis and drug-stimulated apoptosis.
- To create a DNA logic-gated nanoplatform for sensitive aptasensing of ATP on cell surfaces.
Main Methods:
- Engineered triplex-boosted G-quadruplexes (tb-G4s) responsive to H+ and K+.
- Constructed a DNA logic-gated nanoplatform for proximity ATP aptasensing.
- Utilized the platform to capture endogenous ATP released during chemotherapy drug stimulation.
Main Results:
- Demonstrated real-time monitoring of ATP exocytosis from apoptotic cells.
- Successfully captured released ATP to indicate apoptosis progression.
- Provided a sensitive approach for dynamic evaluation of drug-induced apoptosis.
Conclusions:
- The DNA nanoplatform enables sensitive, real-time monitoring of ATP release and apoptosis.
- This method offers a dynamic approach to evaluate chemotherapy drug efficacy.
- The strategy holds potential for personalized cancer therapy assessment.
