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Updated: Oct 26, 2025

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

A Novel α-Globin Chain Variant, Hb Nanchang [HBA2: c.46G>A, Codon 15 (GGT>AGT) (Gly→Ser)], Detected by

Anping Xu¹, Weidong Chen¹, Weijie Xie¹

¹Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China.

|July 26, 2021

Abstract:

Here we report a new α chain variant accidentally discovered during Hb A_1c measurement by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) that revealed the presence of a variant α chain with a mass of 15155 Da. However, this hemoglobin (Hb) variant cannot be detected by the first-line methods such as cation exchange high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Sanger sequencing confirmed the presence of a heterozygous missense mutation [HBA2: c.46G>A, codon 15 (GGT>AGT), (Gly→Ser)]. The theoretical mass difference (30 Da) due to the substitution of amino acid glycine to serine matched the actual measured mass difference (29 Da). As this is the first report of the mutation, we named it Hb Nanchang after the place of residence of the proband.

Keywords:

Hb A1c Hb Nanchang (HBA2: c.46G>A)Matrix-assisted-laser-desorption ionization-time of flight mass spectrometry (MALDI-TOF)mass spectometry (MS)

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