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Updated: Oct 26, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
Gene expression profiling of DNA methyltransferase genes in Siniperca chuatsi based on transcriptome sequencing
Min Zhou1, Ziwei Zhao2, Jinliang Zhao1
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.
Abstract:
The mandarin fish (Siniperca chuatsi) DNA methyltransferase gene 1 (dnmt1) was highly expressed in the mesonephros, head kidney and gonad, whereas dnmt2 was expressed in most tissues. dnmt3a was highly expressed in the brain and spleen, but dnmt3b was mainly expressed in the brain and head kidney. The genes dnmt1 and dnmt2 were highly expressed in the early stages of embryonic development, and dnmt3a and dnmt3b were expressed later. These genes also showed certain changes after artificial diet acclimation, salinity adaptation and immune stress.
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