Quantification of pathogens and antibiotic resistance genes in backyard and commercial composts

Compost is widely used for gardening. Growers can choose to buy compost from markets or make compost at home. Potential exposure of users to pathogens through composting includes ingesting foodborne pathogens and inhaling airborne pathogens. This study compared the abundances of the genetic markers of five opportunistic foodborne and airborne pathogens in the backyard and commercial composts, as well as an immature swine mortality compost. We found that ttrC of Salmonella enterica and ftsZ of Escherichia coli were absent from all ready-to-use compost samples. In contrast, the genes of airborne pathogens such as groEL2 of Mycobacterium spp., mip of Legionella pneumophila, and gyrB of Pseudomonas aeruginosa were detected in the backyard and commercial composts. The groEL2 gene of Mycobacterium spp. was detected in all samples, including the control soil. The abundance of gyrB of P. aeruginosa was high in the two backyard composts, and it was higher than those in any other compost samples. The relative abundances of ARGs were significantly lower in backyard composts than commercial composts. We found that ftsZ of E. coli co-existed with multiple single-drug resistant ARGs in the immature swine mortality compost. We also found that mip of L. pneumophila and gyrB of P. aeruginosa co-existed with aminoglycoside resistance genes. Our findings suggest that inhaling airborne pathogens may carry more risk than ingesting foodborne pathogens when applying composts.