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High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

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Cellulolytic enzymes production guided by morphology engineering.

Mariane M Buffo¹, André L Z Ferreira¹, Renata M R G Almeida²

¹Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil.

Enzyme and Microbial Technology

|July 27, 2021

Summary

Controlling Aspergillus niger morphology optimizes enzyme production. Pellet formation enhances endoglucanase, while dispersed growth boosts β-glucosidase, crucial for lignocellulosic biomass processing.

Keywords:

(hemi) Cellulases Aspergillus niger Enzymes Filamentous fungi Morphology engineering

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GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

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Published on: October 24, 2011

Area of Science:

Biotechnology
Enzymology
Microbial Physiology

Background:

Endoglucanase and xylanase are vital enzymes for lignocellulosic biomass hydrolysis.
Optimizing enzyme secretion by Aspergillus niger is key for efficient biomass conversion.
Microbial morphology can influence enzyme production and secretion.

Purpose of the Study:

To investigate how spore concentration and pH affect Aspergillus niger morphology.
To determine the relationship between A. niger morphology and the production of endoglucanase, xylanase, and β-glucosidase.
To develop a method for directing enzyme selectivity through morphological control.

Main Methods:

Cultivating Aspergillus niger under varying spore concentrations and pH levels.
Analyzing microbial morphology using a form factor-based morphologic scale (Y).
Quantifying endoglucanase, xylanase, and β-glucosidase activities in fermentation broths.

Main Results:

Xylanase production remained unaffected by changes in A. niger morphology.
Endoglucanase production was enhanced in pellet morphology under lower stress conditions.
Dispersed morphology significantly increased β-glucosidase production (up to fourfold).

Conclusions:

A. niger morphology can be manipulated to selectively enhance the production of specific enzymes.
The proposed morphologic scale and equation offer a tool for optimizing enzyme yields for biomass processing.
Morphological characteristics serve as an indicator for relative enzyme activities in fermentation.