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Related Experiment Video

Updated: Oct 26, 2025

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Helminth Microbiota Profiling Using Bacterial 16S rRNA Gene Amplicon Sequencing: From Sampling to Sequence Data

Fabio Formenti1,2, Gabriel Rinaldi3, Cinzia Cantacessi4

  • 1Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|July 27, 2021
PubMed
Summary

Helminth microbiomes are crucial for animal biology but remain understudied. This work details methods for analyzing these microbial communities in parasitic worms like Schistosoma mansoni.

Keywords:
Bacterial 16S rRNA geneBioinformaticsHelminthHigh-throughput sequencingIndirect life cycleSchistosoma mansoniWorm-associated microbiome

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Area of Science:

  • Microbiology
  • Parasitology
  • Animal Biology

Background:

  • Symbiont microbial communities are integral to metazoan organisms, including parasitic helminths.
  • Research on helminth microbiomes and their symbiotic roles is limited, with few parasitic worms studied.
  • Advances in sequencing technologies have increased interest in helminth-associated microbial communities.

Purpose of the Study:

  • To provide an overview of protocols for collecting samples for helminth microbiome analysis.
  • To outline a workflow for characterizing microbial communities associated with parasitic worms.
  • To enhance understanding of helminth biology, physiology, and host-microbe interactions.

Main Methods:

  • Sample collection protocols for helminth microbiome studies.
  • High-throughput sequencing technologies for microbial community characterization.
  • Bioinformatics analyses for large-scale sequence data from helminth-associated microbes.

Main Results:

  • Established protocols for sample collection and processing.
  • Demonstrated a workflow for analyzing helminth-associated microbial communities.
  • Provided a foundation for future research on helminth-microbiome interactions.

Conclusions:

  • Characterizing helminth microbiomes is essential for understanding helminth biology.
  • Standardized protocols and workflows facilitate microbiome research in parasitic worms.
  • This work supports further investigation into the complex relationships between helminths and their symbionts.