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Updated: Oct 26, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Something old, something new, a DNA preparation procedure for long-read genomic sequencing
Hongjun Wang1,1, Sen Xu1,1
1Department of Biology, University of Texas at Arlington, TX 76019, USA.
Abstract:
Extracting high-molecular-weight DNA is critical for successful long-read DNA sequencing. Here, we present a DNA preparation procedure combining the CTAB DNA extraction protocol and a short-read eliminator kit, which consistently produces high yields of high-molecular-weight DNA in the freshwater microcrustacean Daphnia. This method only requires common chemicals and equipment, providing researchers a viable alternative to DNA extraction kits that often require specialized tools.
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