Opportunities and challenges in microbial medium chain fatty acids production from waste biomass

Medium chain fatty acids (MCFAs) that produced from affordable waste biomass via chain elongation (CE) technology are recognized as the potential alternatives to part fossil-derived chemicals, contributing to the sustainable development of economy and environment. The purpose of this review is to provide comprehensive analyses on the opportunities and challenges of MCFAs production and application. First, both two microbial MCFAs synthesis pathways of reverse β-oxidation and fatty acid biosynthesis were introduced/compared in detail to give readers a thorough understanding of the CE process, with the expectation of further boosting MCFAs production by well distinguishing them. Furthermore, the six key MCFAs production bottlenecks, corresponding research progresses, and possible solutions were analyzed. Five major MCFAs production strategies with their production mechanism, performances, and characteristics were also critically assessed. Additionally, the commercial production status was introduced, and future alternative production mode and research priorities were also recommended.