Ecdysteroid excretion by adult Hymenolepis diminuta in vitro

Both patent and prepatent adult Hymenolepis diminuta excreted 20-hydroxyecdysone into the culture medium when maintained in vitro. Patent worms also excreted ecdysone and comparatively large quantities of unidentified immunoreactive material of a relatively apolar nature. This latter material was shown to be depleted from the endogenous free ecdysteroids of patent adults during the culture period. Ecdysteroid excretion was affected both qualitatively and quantitatively when culturing conditions were varied.