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MicroRNA-802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway

L F Gao1, S Jia2, Q M Zhang3

  • 1Department of Clinical Laboratory, Weifang Weiyi Tumor Hospital, Affiliated Hospital of Weifang Medical University, Weifang, 261061, China.

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
|July 28, 2021
PubMed
Abstract

Insights

MicroRNA-802 (miR-802) promotes osteosarcoma (OS) progression by targeting p27 Kip1 (p27), driving migration, invasion, and epithelial-mesenchymal transition (EMT). This miR-802/p27 axis inactivates the PI3K/AKT pathway, offering potential therapeutic targets for OS.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are increasingly implicated in tumor development.
  • Dysregulation of specific miRNAs, like miR-802, is observed in various cancers, including osteosarcoma (OS).
  • Understanding the role of miR-802 in OS pathogenesis is crucial for identifying novel therapeutic strategies.

Purpose of the Study:

  • To investigate the role of miR-802 in osteosarcoma (OS).
  • To evaluate the effect of miR-802 on osteosarcoma cell migration, invasion, and epithelial-mesenchymal transition (EMT).
  • To determine if miR-802 influences the expression or function of p27 Kip1 (p27) in OS and its impact on the PI3K/AKT pathway.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to measure miR-802 and p27 expression in OS tissues and cells.
  • Transwell assays to assess the migratory and invasive capabilities of OS cells.
  • Western blot analysis to detect proteins involved in the PI3K/AKT pathway and EMT.
  • Luciferase reporter assays to confirm p27 as a direct target of miR-802.

Main Results:

  • MiR-802 expression was significantly elevated in OS tissues and correlated with poor clinical outcomes.
  • Overexpression of miR-802 enhanced OS cell migration, invasion, and EMT.
  • p27 was identified as a direct target of miR-802, and its restoration counteracted the pro-migratory, pro-invasive, and pro-EMT effects of miR-802.
  • MiR-802 overexpression led to the inactivation of the PI3K/AKT pathway by targeting p27 in OS cells.

Conclusions:

  • MiR-802 promotes osteosarcoma progression, including EMT, migration, and invasion, by directly targeting p27.
  • The newly identified miR-802/p27/PI3K/AKT signaling axis represents a promising therapeutic target for osteosarcoma treatment.
Keywords:
InvasionMiR-802MigrationOsteosarcomap27

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